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superscript cdna synthesis kit  (Bio-Rad)


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    Structured Review

    Bio-Rad superscript cdna synthesis kit
    Superscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 47989 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+cdna+synthesis+kit/iScript+cDNA+Synthesis+Kit/10__1016_slash_j__isci__2025__114385-339-30-34
    Average 99 stars, based on 47989 article reviews
    superscript cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Ketogenic Diet Reduces Age-Induced Chronic Neuroinflammation in Mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research), cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad), and qPCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    Article Title: A ketogenic diet reduces age-induced chronic neuroinflammation in mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research) and cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    cDNA Synthesis:

    Article Title: Ketogenic Diet Reduces Age-Induced Chronic Neuroinflammation in Mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research), cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad), and qPCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    Article Title: CCL20-CCR6 signaling alters the metabolic reprogramming to promote the pathogenic Th17 cell differentiation
    Article Snippet: .. 23 Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) 24 Cells were harvested, and total RNA was prepared using Trizol reagent (Invitrogen). cDNA was synthesized 25 using oligo (dT)11-16 primer and Superscript cDNA synthesis kit (BioRad). .. Specific gene expression was 26 performed using the SYBR green PCR kit (Qiagen) according to the manufacturer's protocol.

    Article Title: Ketone body metabolism declines with age in mice in a sex-dependent manner
    Article Snippet: Snap frozen tissues were homogenized using RiboZol RNA Extraction Reagent (VWR; N580) and Next Advance Bullet Blender (BBY24M). .. RNA was extracted using the Qiagen RNEasy Mini Kit (Qiagen Cat. 169012183) and checked for purity using Thermo Scientific Nanodrop 2000 (Serial No. D631). cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (BioRad Cat. 1708891) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (BioRad Cat. 1725121) in BioRad CFX96 Real-Time System. ..

    Article Title: A ketogenic diet reduces age-induced chronic neuroinflammation in mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research) and cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    Article Title: Hyaluronic Acid Hydrogel-Based Oral Delivery of Iron Supplemented with Probiotic and Prebiotic Ameliorates Iron-Deficiency Anaemia.
    Article Snippet: The oral iron supplementation leads to gut dysbiosis, further activating intestinal inflammatory response leading to inflammatory bowel syndrome.. Iron salt supplementation combined with synbiotics has become an effective strategy to tackle gut health with iron-deficiency anaemia.. Progressing toward targeted and sustained release of oral drug delivery, in this work, we have developed thiolated-hyaluronic acid encapsulated synbiotic hydrogel (HASH + BIDF) comprising iron dextran (ID), soluble millet dietary fiber (DF), and probiotic Lactobacillus rhamnosus (LR).

    Article Title: Liver Phosphoenolpyruvate Carboxykinase-1 Downregulation via siRNA-Functionalized Graphene Oxide Nanosheets Restores Glucose Homeostasis in a Type 2 Diabetes Mellitus In Vivo Model.
    Article Snippet: Metabolic disorders have been increasing at an alarming rate, and one such example of metabolic disorder is type 2 diabetes mellitus (T2DM).. Unregulated gluconeogenesis in T2DM results in increased hepatic glucose output that causes fasting and postprandial hyperglycaemia.. Extensive proofs have shown that the downregulation of the key rate-limiting enzyme phosphoenolpyruvate carboxykinase-1 (PCK-1) of gluconeogenesis improved glucose homeostasis in vivo.

    Article Title: pH-Triggered, Synbiotic Hydrogel Beads for In Vivo Therapy of Iron Deficiency Anemia and Reduced Inflammatory Response.
    Article Snippet: Iron deficiency anemia (IDA) is the most common nutritional disorder worldwide nearly affecting two billion people.. The efficacies of conventional oral iron supplements are mixed, intravenous iron administration acquaintances with finite but crucial risks.. Usually, only 5−20% iron is absorbed in the duodenum while the remaining fraction reaches the colon, affecting the gut microbes and can significantly impact intestinal inflammatory responses.

    Real-time Polymerase Chain Reaction:

    Article Title: Ketogenic Diet Reduces Age-Induced Chronic Neuroinflammation in Mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research), cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad), and qPCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    Article Title: Ketone body metabolism declines with age in mice in a sex-dependent manner
    Article Snippet: Snap frozen tissues were homogenized using RiboZol RNA Extraction Reagent (VWR; N580) and Next Advance Bullet Blender (BBY24M). .. RNA was extracted using the Qiagen RNEasy Mini Kit (Qiagen Cat. 169012183) and checked for purity using Thermo Scientific Nanodrop 2000 (Serial No. D631). cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (BioRad Cat. 1708891) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (BioRad Cat. 1725121) in BioRad CFX96 Real-Time System. ..

    Article Title: A ketogenic diet reduces age-induced chronic neuroinflammation in mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research) and cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    SYBR Green Assay:

    Article Title: Ketogenic Diet Reduces Age-Induced Chronic Neuroinflammation in Mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research), cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad), and qPCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    Article Title: Ketone body metabolism declines with age in mice in a sex-dependent manner
    Article Snippet: Snap frozen tissues were homogenized using RiboZol RNA Extraction Reagent (VWR; N580) and Next Advance Bullet Blender (BBY24M). .. RNA was extracted using the Qiagen RNEasy Mini Kit (Qiagen Cat. 169012183) and checked for purity using Thermo Scientific Nanodrop 2000 (Serial No. D631). cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (BioRad Cat. 1708891) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (BioRad Cat. 1725121) in BioRad CFX96 Real-Time System. ..

    Article Title: A ketogenic diet reduces age-induced chronic neuroinflammation in mice
    Article Snippet: .. RNA was isolated by Quick-RNA MicroPrep Kit (R1051; Zymo Research) and cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (1725121; BioRad) in a BioRad CFX96 Real-Time System. ..

    Article Title: Hyaluronic Acid Hydrogel-Based Oral Delivery of Iron Supplemented with Probiotic and Prebiotic Ameliorates Iron-Deficiency Anaemia.
    Article Snippet: The oral iron supplementation leads to gut dysbiosis, further activating intestinal inflammatory response leading to inflammatory bowel syndrome.. Iron salt supplementation combined with synbiotics has become an effective strategy to tackle gut health with iron-deficiency anaemia.. Progressing toward targeted and sustained release of oral drug delivery, in this work, we have developed thiolated-hyaluronic acid encapsulated synbiotic hydrogel (HASH + BIDF) comprising iron dextran (ID), soluble millet dietary fiber (DF), and probiotic Lactobacillus rhamnosus (LR).

    Article Title: pH-Triggered, Synbiotic Hydrogel Beads for In Vivo Therapy of Iron Deficiency Anemia and Reduced Inflammatory Response.
    Article Snippet: Iron deficiency anemia (IDA) is the most common nutritional disorder worldwide nearly affecting two billion people.. The efficacies of conventional oral iron supplements are mixed, intravenous iron administration acquaintances with finite but crucial risks.. Usually, only 5−20% iron is absorbed in the duodenum while the remaining fraction reaches the colon, affecting the gut microbes and can significantly impact intestinal inflammatory responses.

    Reverse Transcription:

    Article Title: CCL20-CCR6 signaling alters the metabolic reprogramming to promote the pathogenic Th17 cell differentiation
    Article Snippet: .. 23 Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) 24 Cells were harvested, and total RNA was prepared using Trizol reagent (Invitrogen). cDNA was synthesized 25 using oligo (dT)11-16 primer and Superscript cDNA synthesis kit (BioRad). .. Specific gene expression was 26 performed using the SYBR green PCR kit (Qiagen) according to the manufacturer's protocol.

    Polymerase Chain Reaction:

    Article Title: CCL20-CCR6 signaling alters the metabolic reprogramming to promote the pathogenic Th17 cell differentiation
    Article Snippet: .. 23 Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) 24 Cells were harvested, and total RNA was prepared using Trizol reagent (Invitrogen). cDNA was synthesized 25 using oligo (dT)11-16 primer and Superscript cDNA synthesis kit (BioRad). .. Specific gene expression was 26 performed using the SYBR green PCR kit (Qiagen) according to the manufacturer's protocol.

    Synthesized:

    Article Title: CCL20-CCR6 signaling alters the metabolic reprogramming to promote the pathogenic Th17 cell differentiation
    Article Snippet: .. 23 Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) 24 Cells were harvested, and total RNA was prepared using Trizol reagent (Invitrogen). cDNA was synthesized 25 using oligo (dT)11-16 primer and Superscript cDNA synthesis kit (BioRad). .. Specific gene expression was 26 performed using the SYBR green PCR kit (Qiagen) according to the manufacturer's protocol.

    Article Title: Liver Phosphoenolpyruvate Carboxykinase-1 Downregulation via siRNA-Functionalized Graphene Oxide Nanosheets Restores Glucose Homeostasis in a Type 2 Diabetes Mellitus In Vivo Model.
    Article Snippet: Metabolic disorders have been increasing at an alarming rate, and one such example of metabolic disorder is type 2 diabetes mellitus (T2DM).. Unregulated gluconeogenesis in T2DM results in increased hepatic glucose output that causes fasting and postprandial hyperglycaemia.. Extensive proofs have shown that the downregulation of the key rate-limiting enzyme phosphoenolpyruvate carboxykinase-1 (PCK-1) of gluconeogenesis improved glucose homeostasis in vivo.

    Membrane:

    Article Title: Hyaluronic Acid Hydrogel-Based Oral Delivery of Iron Supplemented with Probiotic and Prebiotic Ameliorates Iron-Deficiency Anaemia.
    Article Snippet: The oral iron supplementation leads to gut dysbiosis, further activating intestinal inflammatory response leading to inflammatory bowel syndrome.. Iron salt supplementation combined with synbiotics has become an effective strategy to tackle gut health with iron-deficiency anaemia.. Progressing toward targeted and sustained release of oral drug delivery, in this work, we have developed thiolated-hyaluronic acid encapsulated synbiotic hydrogel (HASH + BIDF) comprising iron dextran (ID), soluble millet dietary fiber (DF), and probiotic Lactobacillus rhamnosus (LR).

    Article Title: pH-Triggered, Synbiotic Hydrogel Beads for In Vivo Therapy of Iron Deficiency Anemia and Reduced Inflammatory Response.
    Article Snippet: Iron deficiency anemia (IDA) is the most common nutritional disorder worldwide nearly affecting two billion people.. The efficacies of conventional oral iron supplements are mixed, intravenous iron administration acquaintances with finite but crucial risks.. Usually, only 5−20% iron is absorbed in the duodenum while the remaining fraction reaches the colon, affecting the gut microbes and can significantly impact intestinal inflammatory responses.

    Cell Culture:

    Article Title: Liver Phosphoenolpyruvate Carboxykinase-1 Downregulation via siRNA-Functionalized Graphene Oxide Nanosheets Restores Glucose Homeostasis in a Type 2 Diabetes Mellitus In Vivo Model.
    Article Snippet: Metabolic disorders have been increasing at an alarming rate, and one such example of metabolic disorder is type 2 diabetes mellitus (T2DM).. Unregulated gluconeogenesis in T2DM results in increased hepatic glucose output that causes fasting and postprandial hyperglycaemia.. Extensive proofs have shown that the downregulation of the key rate-limiting enzyme phosphoenolpyruvate carboxykinase-1 (PCK-1) of gluconeogenesis improved glucose homeostasis in vivo.

    RNA Extraction:

    Article Title: Liver Phosphoenolpyruvate Carboxykinase-1 Downregulation via siRNA-Functionalized Graphene Oxide Nanosheets Restores Glucose Homeostasis in a Type 2 Diabetes Mellitus In Vivo Model.
    Article Snippet: Metabolic disorders have been increasing at an alarming rate, and one such example of metabolic disorder is type 2 diabetes mellitus (T2DM).. Unregulated gluconeogenesis in T2DM results in increased hepatic glucose output that causes fasting and postprandial hyperglycaemia.. Extensive proofs have shown that the downregulation of the key rate-limiting enzyme phosphoenolpyruvate carboxykinase-1 (PCK-1) of gluconeogenesis improved glucose homeostasis in vivo.

    Agarose Gel Electrophoresis:

    Article Title: Liver Phosphoenolpyruvate Carboxykinase-1 Downregulation via siRNA-Functionalized Graphene Oxide Nanosheets Restores Glucose Homeostasis in a Type 2 Diabetes Mellitus In Vivo Model.
    Article Snippet: Metabolic disorders have been increasing at an alarming rate, and one such example of metabolic disorder is type 2 diabetes mellitus (T2DM).. Unregulated gluconeogenesis in T2DM results in increased hepatic glucose output that causes fasting and postprandial hyperglycaemia.. Extensive proofs have shown that the downregulation of the key rate-limiting enzyme phosphoenolpyruvate carboxykinase-1 (PCK-1) of gluconeogenesis improved glucose homeostasis in vivo.



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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control